| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.223659 |
| Chromosome: | chromosome 12 |
| Location: | 7657450 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g554450 | 3'UTR|CDS|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGCTGCCGGTGCTTGCCGCGCCGCAGCTG |
| Internal bar code: | CTTGGCCATCATACCCGATGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 215 |
| LEAP-Seq percent confirming: | 99.0119 |
| LEAP-Seq n confirming: | 5411 |
| LEAP-Seq n nonconfirming: | 54 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCTATCAACTGCTTCCACG |
| Suggested primer 2: | GCTTCCTGTCTCATTCGAGG |