| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.223688 |
| Chromosome: | chromosome 6 |
| Location: | 7750679 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g302150 | RLS12 | (1 of 587) 2.7.11.1 - Non-specific serine/threonine protein kinase / Threonine-specific protein kinase; RegA/Rls-like protein 12 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGTATCAGTGCTGTACAATAATGCCAGAG |
| Internal bar code: | GTAATGTGGGCATTGCCCCGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 225 |
| LEAP-Seq percent confirming: | 48.9031 |
| LEAP-Seq n confirming: | 1226 |
| LEAP-Seq n nonconfirming: | 1281 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCTGGCGTCTCTGTCTGCT |
| Suggested primer 2: | CTGGAGAGTGCAGCCTTCTT |