Insertion junction: LMJ.RY0402.223753_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.223753
Chromosome:chromosome 7
Location:782964
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g318050 (1 of 10) PF00170 - bZIP transcription factor (bZIP_1) 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATACAACTCGCTAGCTGAAGCGCTTAACAA
Internal bar code:ACGCGACAGACACCTAGCTGAC

Confirmation - LEAP-Seq

LEAP-Seq distance:565
LEAP-Seq percent confirming:87.9032
LEAP-Seq n confirming:109
LEAP-Seq n nonconfirming:15
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:CTGACAAGCTAGGGTGAGGC
Suggested primer 2:GACGAGTCGCTTTAAGGTGC