Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.223755 |
Chromosome: | chromosome_1 |
Location: | 3953482 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre01.g025750 | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | ACCTCGCCCGCAACTCAGACGCGACCACGC |
Internal bar code: | ATCGTGTCACTGGGAGCTTTCG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1231 |
LEAP-Seq percent confirming: | 98.8675 |
LEAP-Seq n confirming: | 19468 |
LEAP-Seq n nonconfirming: | 223 |
LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GACTTCTACGCACACCAGCA |
Suggested primer 2: | GATTAGAGGGAGGGAGCCAC |