| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.223776 |
| Chromosome: | chromosome 10 |
| Location: | 4203317 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g449950 | (1 of 1) PTHR14593:SF5 - WD REPEAT-CONTAINING PROTEIN 11 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGCGATGGCCGCGCCGGTCCCCGCCATC |
| Internal bar code: | GGAGACAGGGCTGTTCGGCTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 32 |
| LEAP-Seq percent confirming: | 82.3529 |
| LEAP-Seq n confirming: | 56 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAGTGGTGAAGCAGTGGGT |
| Suggested primer 2: | ACAGTTCGACACGCTTCCTT |