| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.223795 |
| Chromosome: | chromosome 12 |
| Location: | 5881637 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g533900 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAAGGCCTGGTAACGTGCTGCGTGCAGCA |
| Internal bar code: | GATGGTGGCAGGCTGTGACGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 877 |
| LEAP-Seq percent confirming: | 99.2885 |
| LEAP-Seq n confirming: | 9070 |
| LEAP-Seq n nonconfirming: | 65 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGGAGCTTGCTATAAGCCG |
| Suggested primer 2: | TTGTGAGCTTGACGCATTTC |