Insertion junction: LMJ.RY0402.223797_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.223797
Chromosome:chromosome 7
Location:223041
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre07.g313650 (1 of 70) 4.6.1.1 - Adenylate cyclase / ATP pyrophosphate-lyase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGAGAACGCCTGCGCGCAGGCCGCCGCCG
Internal bar code:GTAGGGGTAATGCATTTGACTC

Confirmation - LEAP-Seq

LEAP-Seq distance:150
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CATTTCCCGACCCATATCAC
Suggested primer 2:CAGTGGTACGCTAAGGAGGC