Insertion junction: LMJ.RY0402.223817_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.223817
Chromosome:chromosome 7
Location:2104251
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g326150 (1 of 34) IPR000571 - Zinc finger, CCCH-type 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGTTTTGAGGAAGACATGTGATGCGACGGT
Internal bar code:TAGATATGCAGGAAGGATCAAA

Confirmation - LEAP-Seq

LEAP-Seq distance:663
LEAP-Seq percent confirming:97.6838
LEAP-Seq n confirming:1687
LEAP-Seq n nonconfirming:40
LEAP-Seq n unique pos:48

Suggested primers for confirmation by PCR

Suggested primer 1:CTTGCCACTCACTCCCTAGC
Suggested primer 2:CCGCCTTTCTTTTCACTCTG