| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.223850 |
| Chromosome: | chromosome 8 |
| Location: | 1698219 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g365825 | (1 of 1) 1.14.99.42 - Zeaxanthin 7,8-dioxygenase / Zeaxanthin 7,8(7',8')-cleavage dioxygenase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGGAGAAGTGAGTGGGGCATGCACGCAA |
| Internal bar code: | CAGGGGGTAGTTCGGGTGTTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 444 |
| LEAP-Seq percent confirming: | 99.7414 |
| LEAP-Seq n confirming: | 10415 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACGGGTGTGTGTGTGTGTA |
| Suggested primer 2: | AAGCTGTATGGCGCAGAGAT |