Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.223866 |
Chromosome: | chromosome_8 |
Location: | 1355526 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre08.g364000 | sense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TGGGTGCCCAGCATCAGCCTTGGTAGCCTT |
Internal bar code: | GAACCAAATTGTTCCTGGGCAA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 170 |
LEAP-Seq percent confirming: | 92.5926 |
LEAP-Seq n confirming: | 150 |
LEAP-Seq n nonconfirming: | 12 |
LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GGTGATGTGCCCTTGCTAAT |
Suggested primer 2: | GAAGCACACACTGGAGACGA |