Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.223870 |
Chromosome: | chromosome_6 |
Location: | 3761262 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre06.g278157 | antisense | 5'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TTGGCGAAGTTCGCCTCGTTTTGGTCTGGG |
Internal bar code: | TGATTTTACCTTCTGATACTAC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 956 |
LEAP-Seq percent confirming: | 95.4549 |
LEAP-Seq n confirming: | 12538 |
LEAP-Seq n nonconfirming: | 597 |
LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ACACATAAACTGCACCGCAA |
Suggested primer 2: | ATGTATGTGGGCCATTGCTT |