| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.223872 |
| Chromosome: | chromosome 12 |
| Location: | 8371664 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g549150 | (1 of 781) IPR000104 - Antifreeze protein, type I | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAGCGCGCCACCCAGCAGGTTGTGGTTGA |
| Internal bar code: | CCGCTCCGGGGTACGGATGTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 606 |
| LEAP-Seq percent confirming: | 86.3134 |
| LEAP-Seq n confirming: | 1873 |
| LEAP-Seq n nonconfirming: | 297 |
| LEAP-Seq n unique pos: | 42 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAAGCAAGAGGTTGCAAAG |
| Suggested primer 2: | TGTTTCGTCCTGCTCATCTG |