| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.223896 |
| Chromosome: | chromosome_17 |
| Location: | 3895254 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre17.g728300 | FAP226,CAL6 | Calpain cysteine protease and Flagellar Associated Protein | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CCTAGCCCAATGGGACCGAGGCCCAGGGGG |
| Internal bar code: | TCATCGGTAGCAGTATCTATCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 353 |
| LEAP-Seq percent confirming: | 99.4407 |
| LEAP-Seq n confirming: | 4089 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGTGGATAAGCATACGGCG |
| Suggested primer 2: | GAGTACATGGTGGTGGGGTC |