| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.223941 |
| Chromosome: | chromosome 8 |
| Location: | 3388301 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g374350 | (1 of 6) PF01847 - von Hippel-Lindau disease tumour suppressor protein (VHL) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATACAACCGTAGCAGGATATGGGCATGGG |
| Internal bar code: | CAAGACGCACCGGGACGTGACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 759 |
| LEAP-Seq percent confirming: | 98.9592 |
| LEAP-Seq n confirming: | 3613 |
| LEAP-Seq n nonconfirming: | 38 |
| LEAP-Seq n unique pos: | 53 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGTGGGTTCTGCAATACTT |
| Suggested primer 2: | GGGACTGCCACCGTAGTTTA |