| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.224025 |
| Chromosome: | chromosome_6 |
| Location: | 3784729 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre06.g278162 | PHC3 | Cell wall protein pherophorin-C3 | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | AAAAGCGAGTCCAGCACACATGTGTAGTCC |
| Internal bar code: | GGCTCTCAGCCGCGGATCGGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 628 |
| LEAP-Seq percent confirming: | 98.3168 |
| LEAP-Seq n confirming: | 993 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 36 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGGAGGGCAGTCTGCAGTT |
| Suggested primer 2: | CAGCGTTCTGAACAAGACCA |