Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.224043 |
Chromosome: | chromosome_10 |
Location: | 4132120 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre10.g449750 | PKS1 | sense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GTCGGACATCGCTGGGTCCGTGGCGGCGCT |
Internal bar code: | AAAGGATGCCAGAACAGGGGGC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 482 |
LEAP-Seq percent confirming: | 97.5664 |
LEAP-Seq n confirming: | 441 |
LEAP-Seq n nonconfirming: | 11 |
LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CTTCACTCTCGGTGGGACAT |
Suggested primer 2: | TGCTGCAAGCTGCTGTAAAC |