Insertion junction: LMJ.RY0402.224068_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.224068
Chromosome:chromosome 6
Location:3467906
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g278095 (1 of 1) IPR016024//IPR027417 - Armadillo-type fold // P-loop containing nucleoside triphosphate hydrolase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCCGCCTCCGCCAGCGCCTTCATCAGCTC
Internal bar code:ATGAATGGTAGAGGCGCCCTGC

Confirmation - LEAP-Seq

LEAP-Seq distance:118
LEAP-Seq percent confirming:25.4237
LEAP-Seq n confirming:30
LEAP-Seq n nonconfirming:88
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:CGTGGTGATCAGTATGTGCC
Suggested primer 2:AAATACGCAACAGGGACGAC