| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.224074 |
| Chromosome: | chromosome 6 |
| Location: | 1667150 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g261050 | (1 of 28) 2.7.7.6 - DNA-directed RNA polymerase / RNA polymerase III | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCACAAGGGCAAAGACGCTTTCCCCCACCC |
| Internal bar code: | TCAGAATCGCGTGGCGCGGACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 466 |
| LEAP-Seq percent confirming: | 98.4816 |
| LEAP-Seq n confirming: | 454 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 42 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTCCACACACACACACACA |
| Suggested primer 2: | CATCTTTGTTCCCCGCTTTA |