| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.224105 |
| Chromosome: | chromosome 1 |
| Location: | 5904073 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g042050 | MSRB3 | Methionine sulphoxide reductase; (1 of 3) K07305 - peptide-methionine (R)-S-oxide reductase (msrB) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCGGGCCCACGGCTTCCAAACATGCTCCG |
| Internal bar code: | CGTGAGGTTTTGATCCATAGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 362 |
| LEAP-Seq percent confirming: | 78.6325 |
| LEAP-Seq n confirming: | 92 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAGACCTTAGAGGCAGCAC |
| Suggested primer 2: | GGGATTAGTGTCCACACGCT |