| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.224134 |
| Chromosome: | chromosome 12 |
| Location: | 5150444 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g527150 | (1 of 1) PF09234 - Domain of unknown function (DUF1963) (DUF1963) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGACACCCGGCACTCCGCGCCACTCTCGC |
| Internal bar code: | GGTAGCGACGAGCCACCAGCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 854 |
| LEAP-Seq percent confirming: | 97.502 |
| LEAP-Seq n confirming: | 1249 |
| LEAP-Seq n nonconfirming: | 32 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCGCAAGTGAATTCTAACA |
| Suggested primer 2: | ACGTGCGTAATCTGCTTGTG |