Insertion junction: LMJ.RY0402.224141_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.224141
Chromosome:chromosome 16
Location:6032739
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre16.g676350 (1 of 1) PTHR23073//PTHR23073:SF37 - 26S PROTEASE REGULATORY SUBUNIT // SUBFAMILY NOT NAMED intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCACCCCTACACAGCTACCACACCAAACCC
Internal bar code:CCCTTGGATTTCCGGGCGGGAA

Confirmation - LEAP-Seq

LEAP-Seq distance:0
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:ATGGAACGTTTGCCTAATGC
Suggested primer 2:CCTTATCTGATGTGCGACGA