| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.224157 |
| Chromosome: | chromosome 7 |
| Location: | 1675713 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g325450 | (1 of 4) PF06966 - Protein of unknown function (DUF1295) (DUF1295) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTGGGCTGTAAAGTGTACATGAGGTTTGT |
| Internal bar code: | ATGGAAGGTCAGGGGACAGCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 749 |
| LEAP-Seq percent confirming: | 98.8206 |
| LEAP-Seq n confirming: | 9217 |
| LEAP-Seq n nonconfirming: | 110 |
| LEAP-Seq n unique pos: | 138 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAACACGCTGTGCTTTTTC |
| Suggested primer 2: | TCTCAGGCCAAAACTACGCT |