| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.224165 |
| Chromosome: | chromosome 1 |
| Location: | 6295124 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g044850 | (1 of 1) K17592 - sacsin (SACS) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGCCGCCAGGATTCCGGGGCCGTCACAGG |
| Internal bar code: | CCGTGATCAGGAGCCGAGGATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 256 |
| LEAP-Seq percent confirming: | 80.2198 |
| LEAP-Seq n confirming: | 73 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTAGCCGTTTCGTAGATGG |
| Suggested primer 2: | CACACACCTCCACACACCTC |