| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.224165 |
| Chromosome: | chromosome 6 |
| Location: | 8899991 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g311150 | MFT20 | Major facilitator superfamily transporter; (1 of 3) PTHR23505:SF12 - MAJOR FACILITATOR PROTEIN | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAGGTCGTCACAATACCGCGGGGGATGTG |
| Internal bar code: | GGGTCGTAAAGATCGCGGCGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 319 |
| LEAP-Seq percent confirming: | 90.0 |
| LEAP-Seq n confirming: | 378 |
| LEAP-Seq n nonconfirming: | 42 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTGTGAACGTGTAGGGGCT |
| Suggested primer 2: | TAGGAATTTCACCCACCTGC |