| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.224168 |
| Chromosome: | chromosome 10 |
| Location: | 186987 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g419000 | (1 of 1) PTHR13105//PTHR13105:SF7 - MYELOID LEUKEMIA FACTOR // MYELOID LEUKEMIA FACTOR | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGCTTCTCGCTCCGGCTGACATGTTCCC |
| Internal bar code: | ACTCCCTCTGCGAGCGAAGAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1262 |
| LEAP-Seq percent confirming: | 99.2965 |
| LEAP-Seq n confirming: | 10022 |
| LEAP-Seq n nonconfirming: | 71 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACACACACCACACACACG |
| Suggested primer 2: | TCTTACGGGAGGGTACATGC |