Insertion junction: LMJ.RY0402.224194_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.224194
Chromosome:chromosome 6
Location:7163315
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g297700 (1 of 1) IPR013026//IPR019734//IPR027417 - Tetratricopeptide repeat-containing domain // Tetratricopeptide repeat // P-loop containing nucleoside triphosphate hydrolase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTCCCGCTTACCCCCGCGACCACGCCGCG
Internal bar code:GAGACGCGATCGGGGCCATACT

Confirmation - LEAP-Seq

LEAP-Seq distance:626
LEAP-Seq percent confirming:43.8683
LEAP-Seq n confirming:2944
LEAP-Seq n nonconfirming:3767
LEAP-Seq n unique pos:25

Suggested primers for confirmation by PCR

Suggested primer 1:CGCTTCTGCTGTGTTGTGAT
Suggested primer 2:CTAGTAGGGATGGGGGTGGT