| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.224204 |
| Chromosome: | chromosome 2 |
| Location: | 3650953 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g095149 | FAP143 | Flagellar Associated Protein 143 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTCCCACGCCCTTTCGCCGCCGTCCCCCG |
| Internal bar code: | CCTTCTGTGCTCGTTCCAGAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1189 |
| LEAP-Seq percent confirming: | 99.219 |
| LEAP-Seq n confirming: | 3303 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGTGGGCAGCAGAAAGTGT |
| Suggested primer 2: | CTTCAAACAGCAGGAAAGCC |