| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.224231 |
| Chromosome: | chromosome 6 |
| Location: | 3692096 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278142 | (1 of 1) K14441 - ribosomal protein S12 methylthiotransferase [EC:2.8.4.4] (rimO) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCCAGGGGTAACATAAGTATCGGATGCAT |
| Internal bar code: | CTTTCTGACTCGACAATAGAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 453 |
| LEAP-Seq percent confirming: | 98.9097 |
| LEAP-Seq n confirming: | 635 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGGCAATGTGTACATCAAG |
| Suggested primer 2: | AACTGCTTGTCTGTTCGGCT |