| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.224233 |
| Chromosome: | chromosome 12 |
| Location: | 8613368 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g547100 | CGL2,SMM43 | (1 of 2) PTHR10108//PTHR10108:SF788 - METHYLTRANSFERASE // SUBFAMILY NOT NAMED; putative methyltransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGGCGCAAACGGGCCAAACTCGGATAACG |
| Internal bar code: | AAGTTCAATAACATATTGTGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1048 |
| LEAP-Seq percent confirming: | 99.3598 |
| LEAP-Seq n confirming: | 4811 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGATGTCGCTCTCCGTATTT |
| Suggested primer 2: | TTCATGGTCAGAATGGTGGA |