| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.224243 |
| Chromosome: | chromosome 14 |
| Location: | 1040260 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g614500 | BTB/POZ domain containing protein; (1 of 25) IPR000210//IPR011333 - BTB/POZ domain // POZ domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCCCACTTCCCAGTCCGCTTGTTGCTGTT |
| Internal bar code: | GTTGAATCAGGATGCGTCATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 494 |
| LEAP-Seq percent confirming: | 98.1111 |
| LEAP-Seq n confirming: | 9453 |
| LEAP-Seq n nonconfirming: | 182 |
| LEAP-Seq n unique pos: | 77 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGCTAATTTGCATTTGCTG |
| Suggested primer 2: | GTTGAGGGAGTGAGGGGATT |