Insertion junction: LMJ.RY0402.224245_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.224245
Chromosome:chromosome_10
Location:3836348
Confidence (%):95
Locus disrupted Locus common name Defline Orientation Feature
Cre10.g447300 antisense intron

Insertion site details

Flanking sequence (orientation from cassette outwards):CCTGGCGCAAGGCTCAGTGCACGACGCACC
Internal bar code:ACTGATGGCCTATATAGGGCCG

Confirmation - LEAP-Seq

LEAP-Seq distance:542
LEAP-Seq percent confirming:99.17
LEAP-Seq n confirming:2987
LEAP-Seq n nonconfirming:25
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:TTTTCATCATCAGGGCACAG
Suggested primer 2:CCCTACCTGTGATGTGCCTT