Insertion junction: LMJ.RY0402.224246_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.224246
Chromosome:chromosome 2
Location:2730565
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre02.g093700 (1 of 1) K14951 - cation-transporting ATPase 13A3/4/5 (ATP13A3_4_5) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGGTCTCCGCGCGTCCATATCTTCCTCAG
Internal bar code:CTCCACGTCACATTATGGTCCG

Confirmation - LEAP-Seq

LEAP-Seq distance:585
LEAP-Seq percent confirming:99.3192
LEAP-Seq n confirming:1313
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:AAGCTTAGCAAGGGCTTTCC
Suggested primer 2:ATCTATCGCCACCGTAGCTG