| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.224256 |
| Chromosome: | chromosome 3 |
| Location: | 8255721 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g199450 | MINE2 | Chloroplast division site determinant | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCTTCATCCGGGTTGGCTTTGGGTCAGGT |
| Internal bar code: | TGATATCGCCGTTCCCAGAAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1293 |
| LEAP-Seq percent confirming: | 99.418 |
| LEAP-Seq n confirming: | 7174 |
| LEAP-Seq n nonconfirming: | 42 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAGTGTGGATCTTTGCAGC |
| Suggested primer 2: | TGGTACCTGGGTTTTCAAGC |