| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.224258 |
| Chromosome: | chromosome 3 |
| Location: | 8343801 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g198850 | TL19 | (1 of 3) PTHR31407//PTHR31407:SF18 - FAMILY NOT NAMED // PSBP DOMAIN-CONTAINING PROTEIN 4, CHLOROPLASTIC; Thylakoid llumen protein | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTATGTACTTATAAGCAGCTGTGCTCGGC |
| Internal bar code: | CGGTCCTTAGAGTCATTCAGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 605 |
| LEAP-Seq percent confirming: | 97.5806 |
| LEAP-Seq n confirming: | 1936 |
| LEAP-Seq n nonconfirming: | 48 |
| LEAP-Seq n unique pos: | 48 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGATTACAGCGGGTGGTA |
| Suggested primer 2: | CAGGCTCCTGTTCATCCAAT |