Insertion junction: LMJ.RY0402.224262_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.224262
Chromosome:chromosome 3
Location:434877
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre03.g144987 (1 of 587) 2.7.11.1 - Non-specific serine/threonine protein kinase / Threonine-specific protein kinase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCCATGCCCTGACGTGCCTTCCCCTCACT
Internal bar code:AAAACGCCACGGGACTAGGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:372
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:GTGCGTGCATGTACTGTGTG
Suggested primer 2:CACCACCACCACAGCAATAG