| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.224272 |
| Chromosome: | chromosome 7 |
| Location: | 1744339 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g325717 | (1 of 7) 2.4.99.18 - Dolichyl-diphosphooligosaccharide--protein glycotransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCTCTAATGGCGGTGGGCGTGCCGGTGCT |
| Internal bar code: | GGGGGCACAGCGCCACGGGAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1030 |
| LEAP-Seq percent confirming: | 98.5051 |
| LEAP-Seq n confirming: | 3163 |
| LEAP-Seq n nonconfirming: | 48 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAACGAAGACAAGCCCTCT |
| Suggested primer 2: | CTGGTGAGTGGTATTGGCCT |