| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.224278 |
| Chromosome: | chromosome_14 |
| Location: | 3609689 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre14.g631200 | DII6,IC97,FAP94 | Flagellar Associated Protein | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ATCAACATCAGAAAGCCCACGCAGTCACGA |
| Internal bar code: | GAGCTGGTTTGATAAGCGAAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 498 |
| LEAP-Seq percent confirming: | 99.3798 |
| LEAP-Seq n confirming: | 641 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATACGAGCGCTGTTTAGGC |
| Suggested primer 2: | GACATGGTGTGTAGGATGCG |