| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.224285 |
| Chromosome: | chromosome 6 |
| Location: | 8436882 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g307250 | (1 of 1) PTHR18937//PTHR18937:SF240 - STRUCTURAL MAINTENANCE OF CHROMOSOMES SMC FAMILY MEMBER // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTACAGGTGGCTCGTGCTCCTATGGGCCT |
| Internal bar code: | CACATTATCGAAAAACCTCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 943 |
| LEAP-Seq percent confirming: | 99.1131 |
| LEAP-Seq n confirming: | 447 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGTTTGAGAGTTGCTCTGC |
| Suggested primer 2: | GTGTGGGTTGTGGCTGTATG |