Insertion junction: LMJ.RY0402.224285_3

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.224285
Chromosome:chromosome 6
Location:8436894
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g307250 (1 of 1) PTHR18937//PTHR18937:SF240 - STRUCTURAL MAINTENANCE OF CHROMOSOMES SMC FAMILY MEMBER // SUBFAMILY NOT NAMED intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATCACAACGCATTCATACAACTTGAGTGAA
Internal bar code:CGGCGGTGCCGATGGTGGTCCT

Confirmation - LEAP-Seq

LEAP-Seq distance:670
LEAP-Seq percent confirming:99.1261
LEAP-Seq n confirming:3970
LEAP-Seq n nonconfirming:35
LEAP-Seq n unique pos:53

Suggested primers for confirmation by PCR

Suggested primer 1:GGGTTTGAGAGTTGCTCTGC
Suggested primer 2:GTGTGGGTTGTGGCTGTATG