| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.224286 |
| Chromosome: | chromosome 17 |
| Location: | 2197920 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g713200 | OMT2 | (1 of 2) PTHR10283//PTHR10283:SF76 - SOLUTE CARRIER FAMILY 13 MEMBER // DICARBOXYLATE TRANSPORTER 1, CHLOROPLASTIC; Oxoglutarate:malate antiporter | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTCACGCCGCCAGCGCCCTGCGGGCCGCG |
| Internal bar code: | AAGGCTTCTTTGCTTTTTAACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1310 |
| LEAP-Seq percent confirming: | 99.129 |
| LEAP-Seq n confirming: | 5349 |
| LEAP-Seq n nonconfirming: | 47 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCGCACTACTTCTTCGCCT |
| Suggested primer 2: | AAAGCTGAGTCTCCGTGGAA |