| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.224297 |
| Chromosome: | chromosome 8 |
| Location: | 4123578 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g379800 | (1 of 1) PF00207//PF07974 - Alpha-2-macroglobulin family (A2M) // EGF-like domain (EGF_2) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCGCCGTGAACGCTGCCGCTGCTGGCGTT |
| Internal bar code: | GACCAAAAGAGAAGGCAGTTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 636 |
| LEAP-Seq percent confirming: | 91.3934 |
| LEAP-Seq n confirming: | 669 |
| LEAP-Seq n nonconfirming: | 63 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGAGAGCATCAAAATCACA |
| Suggested primer 2: | AGGCGGAAGATGATTGAATG |