| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.224319 |
| Chromosome: | chromosome_17 |
| Location: | 4500469 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre17.g732500 | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CGCCTCTCACTGGCATTGGTACGAACCACG |
| Internal bar code: | CGAGGGAGGTGGACACGTACGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 704 |
| LEAP-Seq percent confirming: | 84.7826 |
| LEAP-Seq n confirming: | 39 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTGATGAGTTTGCGGGTTT |
| Suggested primer 2: | GTCGTGTACGACATGCAACC |