Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.224319 |
Chromosome: | chromosome_17 |
Location: | 4500474 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre17.g732500 | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GCACACGCACCAGAATGCGGAGTCGCCACT |
Internal bar code: | GCCCTCACCGGCGTCTGTCTCA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 743 |
LEAP-Seq percent confirming: | 99.4407 |
LEAP-Seq n confirming: | 5334 |
LEAP-Seq n nonconfirming: | 30 |
LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GTTGATGAGTTTGCGGGTTT |
Suggested primer 2: | GTCGTGTACGACATGCAACC |