Insertion junction: LMJ.RY0402.224325_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.224325
Chromosome:chromosome 6
Location:7266137
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g298400 (1 of 34) IPR001878 - Zinc finger, CCHC-type 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAGTGAGAATGAGAGACCACGGCGCTCAGA
Internal bar code:TAGGGCAGTGCTCTTTGAATCG

Confirmation - LEAP-Seq

LEAP-Seq distance:1001
LEAP-Seq percent confirming:99.132
LEAP-Seq n confirming:3883
LEAP-Seq n nonconfirming:34
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:AATACCAGAAATGATGCGCC
Suggested primer 2:TGATCACTGCGAGTGAGACC