| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.224344 |
| Chromosome: | chromosome 7 |
| Location: | 6142003 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g355850 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAAACGGAAGGACAGGCGCCGACTTGCGG |
| Internal bar code: | TTGCCCACCGAGCAAGTTCCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 256 |
| LEAP-Seq percent confirming: | 98.688 |
| LEAP-Seq n confirming: | 677 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTTTGGGCAAATTTCGTTC |
| Suggested primer 2: | CCCTGCCCTGCAGTATGTAT |