| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.224347 |
| Chromosome: | chromosome 10 |
| Location: | 1200486 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g426292 | PYK6 | (1 of 3) PTHR11817//PTHR11817:SF4 - PYRUVATE KINASE // PYRUVATE KINASE; Pyruvate kinase 6 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCCACCCACCTGACACACACACACACGCG |
| Internal bar code: | CATGGAACTCGATCCGGGATAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 235 |
| LEAP-Seq percent confirming: | 10.3855 |
| LEAP-Seq n confirming: | 264 |
| LEAP-Seq n nonconfirming: | 2278 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACCAGCCCAAACTAAACCG |
| Suggested primer 2: | GCAAACCTTTGGGTCAGGTA |