| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.224350 |
| Chromosome: | chromosome 5 |
| Location: | 3406493 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g241150 | (1 of 1) K11491 - condensin-2 complex subunit D3 (NCAPD3) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACTTCAGCTGTTTTTGGCGCCGTCGCAAA |
| Internal bar code: | AGCCAAGGCAGCACGGCGATAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 221 |
| LEAP-Seq percent confirming: | 85.6383 |
| LEAP-Seq n confirming: | 161 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACCAATCAACCCGCTCTTC |
| Suggested primer 2: | CACCTTCATCGCAGCAGAC |