| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.224402 |
| Chromosome: | chromosome 11 |
| Location: | 2426268 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g475100 | NAT26 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTGGAGCCCATTATCATGTACCGATCTTG |
| Internal bar code: | AGGGGGCACATGGGTTGCCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 657 |
| LEAP-Seq percent confirming: | 97.6391 |
| LEAP-Seq n confirming: | 2440 |
| LEAP-Seq n nonconfirming: | 59 |
| LEAP-Seq n unique pos: | 50 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCAATCTACCATGCTTGCC |
| Suggested primer 2: | ACCGCTGATCCACCTATCAC |