| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.224405 |
| Chromosome: | chromosome 17 |
| Location: | 2523339 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g716350 | GT90F32,GT90-32 | GT90 family protein 32; (1 of 52) PF05686 - Glycosyl transferase family 90 (Glyco_transf_90) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTCTGTACGCAGCGGGCCACACGCTCGCG |
| Internal bar code: | CCGTGGGGCGTTAAATCGTCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 161 |
| LEAP-Seq percent confirming: | 99.0403 |
| LEAP-Seq n confirming: | 1032 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAAGAAGTCCTCCCACTCC |
| Suggested primer 2: | CCCATGTGGCTCTAGTGGTT |