| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.224416 |
| Chromosome: | chromosome 17 |
| Location: | 3313833 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g723250 | CPP1,CPE1 | chloroplast processing enzyme; (1 of 1) PTHR11851//PTHR11851:SF152 - METALLOPROTEASE // PROTEIN UCR-2.3 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGCTTCGTCCCTGTGTCCAGAGGCAACCC |
| Internal bar code: | TGTCGGTGATTCCGCGACGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1035 |
| LEAP-Seq percent confirming: | 98.9637 |
| LEAP-Seq n confirming: | 955 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAACTGGACGTGAACGAGAA |
| Suggested primer 2: | TATTAGAGGCGCCACAGCTT |